The Rio Grande Valley (RGV), which has a predominantly Hispanic population, experiences high rates of obesity, diabetes, and liver diseases that can increase the risk of liver cancer. Environmental factors, such as arsenic contamination in drinking water, together with unhealthy dietary habits like high-fat diets, may contribute to these health outcomes. This project will examine liver tissue samples and use advanced cell models to better understand how these environmental and lifestyle factors affect liver health at the molecular level. By identifying potential links between arsenic exposure, diet, and liver disease progression, the study aims to uncover early signs of liver damage and cancer risk. The findings could support public health efforts to improve water safety, promote healthier lifestyles, and encourage earlier screening for liver disease and liver cancer in the RGV community.